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I9889 Sigma-Aldrich

Anti-Human IgA (α-chain specific) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgA Secondary Antibodies and Conjugates, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   : 2.5-5.0 mg/mL using Antibody concentration by precipitin Vs Human serum
  : 4.0-6.0 mg/mL using Antibody concentration by precipitin Vs Human whey
  Ouchterlony double diffusion: 1:16
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   secondary antibodies
Quality Level   200

Description

General description

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.
Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins.

Immunogen

IgA purified from normal human colostrum

Application

Anti-Human IgA (α-chain specific) antibody is suitable for use in enzyme-linked immunosorbent assay (ELISA), quantitative precipitin assay (2.5-5 mg/mL), immunoelectrophoresis and Ouchterlony double diffusion (1:16).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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