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ISB1L Sigma-Aldrich

InstantBlue

Ultrafast Protein Stain

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Description

Components

contains Coomassie dye, ethanol, phosphoric acid and solubilizing agents in water. (Caution: Phosphoric acid is a corrosive liquid.)

Other Notes

Not available in Sweden and Denmark

General description

InstantBlue is a ready-to-use solution stain that is specially formulated for ultra-fast (less than 15 min), sensitive (5 ng per band (BSA)) and safe detection of your proteins. Protein gels can be stained with InstantBlue in minutes without the need to wash, fix or destain. Gels can remain in the stain for weeks without concern. Only the proteins are stained resulting in well-defined blue bands on a highly transparent background. The reduction of background interference results in a better signal to noise ratio and may also have a positive impact on the overall resolution and sensitivity. The InstantBlue formulation is non-toxic and does not contain any methanol. Proteins stained using the InstantBlue stain are also compatible with mass spectrometry (MS) analysis.

Legal Information

InstantBlue is a trademark of Expedeon Protein Solutions

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Same Products - New Packaging
Protocols & Articles

Articles

IP-Western: Western Blot Troubleshooting

IP-Western analysis remains a popular technique for identifying protein-protein interactions and identifying unknown proteins in a multi-protein complex. The steps include cell lysis, formation of th...
Keywords: Cell disruption, Degradations, Immunoprecipitation, Individual protein Immunoprecipitation, Nucleic acid denaturation, Precipitation, Western blot

Perparing Cell Lysates: Western Blot Troubleshooting

Often, the first step of analyzing protein expression or protein-protein interactions is to obtain a cell or tissue sample, lyse the cells, and extract proteins using extraction reagents. Total prote...
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Gene expression, Homogenization, PAGE, Sonication, Western blot

Sample Preparation & Gel Electrophoresis: Western Blot Troubleshooting

Electric currents, wires, leads, combs, leaks… so many opportunities for trouble. But we still use gels, because electrophoresis remains an effective way to separate proteins — so that the results of...
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Homogenization, PAGE, Sample preparations, Sonication, Western blot

Peer-Reviewed Papers
15

References

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