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ISO2 Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

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Properties

Related Categories Antibodies, Antibody Characterization and Analysis, Antibody Isotyping Kits, Antibody Kits, Antibody Purification & Characterization Kits,
Quality Level   200
usage   sufficient for 1000 tests (clones) (by ELISA)
  sufficient for 40 tests (clones) (by immunodiffusion, ODD)
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA).

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Features and Benefits

• May be used in a variety of assay formats
• Suitable for all antibody forms
• Determines all mouse IgG subclasses, IgA, and IgM

Kit component only

Description

Product #

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goat antisera to mouse IgA .2 mL    
goat antisera to mouse IgG3 .2 mL    
goat antisera to mouse IgG1 .2 mL    
goat antisera to mouse IgG2a .2 mL    
goat antisera to mouse IgG2b .2 mL    
goat antisera to mouse IgM .2 mL    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Procedures

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Capture ELISA, Cell culture, Cell signaling, Enzyme-linked immunosorbent assay, Hormones, Indirect ELISA, Titrations

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Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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