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J3020 Sigma-Aldrich

Anti-JAB1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Jun activation domain-binding protein-1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using sections of human breast carcinoma
  immunoprecipitation (IP): 4-6 μg using RIPA extract (0.5 mg) of cultured rat pheochromocytoma PC12 cells
  microarray: suitable
  western blot: 0.25-0.5 μg/mL using whole extract of cultured mouse NIH3T3 fibroblasts
species reactivity   human, mouse, rat
mol wt   antigen mol wt 38 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q92905
Gene Information   human ... COPS5(10987)
mouse ... Cops5(26754)
rat ... Cops5(312916)

Description

General description

The gene JAB1 (Jun activation domain-binding protein-1) encodes a component of COP9 signalosome that functions in the phosphorylation of specific transcription factors. The gene is mapped to human chromosome 8q13.2. The encoded protein contains 334 amino acids with a molar mass of 38kDa.

Immunogen

synthetic peptide corresponding to amino acid residues 4-20 of human JAB1 with C-terminal added cysteine conjugated to KLH. The sequence is identical in mouse and rat.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The gene JAB1 (Jun activation domain-binding protein-1), also referred to as COPS5 (COP9 subunit 5), is a coactivator of AP-1 (activator protein 1) transcription factor. It is also involved in the regulation of cell cycle by degradation of the cyclin-dependent kinase inhibitor p27Kip1. It increases the levels of endogenous phospho-c-Jun by activating c-Jun amino-terminal kinase (JNK) activity. These activities are inhibited by association with macrophage migration inhibitory factor (MIF), a critical cytokine in several inflammatory responses. This binding, however, does not affect induction of the transcription factor NF (nuclear factor)-κB. It exists as a monomer and functions in deneddylation of NEDD8 (neural precursor cell expressed, developmentally down-regulated 8). It facilitates the shuttling of certain molecules between the nucleus and cytoplasm. JAB1 regulates cellular proliferation and apoptosis by modulating intracellular signaling. Overexpression of this protein has been associated with tumorigenic process.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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