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  • J4500 - Anti-c-Jun N-Terminal Kinase antibody produced in rabbit

J4500 Sigma

Anti-c-Jun N-Terminal Kinase antibody produced in rabbit

whole antiserum

Synonym: Anti-JNK1, JNK2



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Chromatin,
species reactivity   human, mouse, rat
application(s)   microarray: suitable
  western blot: 1:16,000 using rat brain extract and mouse NIH-3T3 fibroblast extract, respectively
  western blot: 1:2,000 using mouse NIH 3T3 fibroblast cell lysate
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen, JNK1 mol wt 46 kDa
  antigen, JNK2 mol wt 55 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK8(5599)
mouse ... Mapk8(26419)
rat ... Mapk8(116554)
biological source   rabbit
conjugate   unconjugated



Synthetic peptide corresponding to amino acids 339-354 of human JNK1 (c-Jun N-terminal kinase 1), conjugated to KLH. The sequence is highly conserved in JNK1, JNK2 α, β, γ (p54 SAPK α, β, γ) and identical in human, rat, and mouse JNK1.

General description

JNK (c-Jun N terminal protein kinase, also known as stress activated protein kinase, SAPK1) is a protein that belongs to serine/threonine-protein kinase family. It has a critical role in inhibiting WOX1 (WW domain-containing oxidoreductase) mediated apoptosis. It also mediates starvation-induced BCL2 phosphorylation and activates autophagy. Anti-c-Jun N-terminal kinase antibody can be used in microarray. Rabbit anti-c-Jun N-terminal kinase antibody reacts specifically with JNK1 (46 kD) and JNK2 (55 kD) of cell culture lysates and rat brain tissue extracts. The product has also shown weak cross reactivity for JNK2β (50 kD) isoform in mouse NIH-3T3 fibroblast cell lysate.


Anti-c-Jun N-terminal kinase antibody can be used in immunoblotting (diluted 1:16,000 or 1:2000) for detecting JNK1 (46kD) and JNK2 (55kD) isoforms using a rat brain extract and a NIH 3T3 mouse fibroblasts cell lysate.

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RIDADR  NONH for all modes of transport
WGK Germany  3
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
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