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  • K0514 - Anti-KCNK9 (TASK-3) antibody, Mouse monoclonal

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K0514 Sigma-Aldrich

Anti-KCNK9 (TASK-3) antibody, Mouse monoclonal

clone KCN-75, purified from hybridoma cell culture

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Ion Channels, Antibodies to Potassium Channels,
conjugate   unconjugated
clone   KCN-75, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 4-8 μg/mL using whole extract of cultured T-47D cells (human breast carcinoma)
species reactivity   human
mol wt   antigen ~40 kDa (representing the KCNK9 monomer, and a weaker band at 80 kDa representing the KCNK9 dimer)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q9NPC2
Gene Information   human ... KCNK9(51305)

Description

General description

Monoclonal Anti- KCNK9 (TASK-3), Clone KCN-75, (mouse IgG2b isotype) is derived from the KCN-75 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Potassium two pore domain channel subfamily K member 9 (KCNK9) gene has been localized to the chromosomal region 8q24.

The gene KCNK9 (potassium two pore domain channel subfamily K member 9), also referred to as TWIK-related acid sensitive K(+) channel-3 (TASK-3), encodes a new member of the K2P (two pore domain K+ channel) family of proteins. It is found to be expressed in several rat tissues, such as brain, kidney, liver, lung, colon, stomach, spleen, testis, and skeletal muscle. It is also expressed to a lesser extent in heart and small intestine. All the potassium channels contain a conserved P domain that is involved in providing K+ selectivity and two, four, or six transmembrane segments.

Immunogen

synthetic peptide corresponding to amino acids 360-374 in the C-terminus of human KCNK9.

Application

Anti-KCNK9 (TASK-3) antibody, Mouse monoclonal has been used in:
• immunoblotting
• immunohistochemistry
• immunocytochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Potassium two pore domain channel subfamily K member 9 (KCNK9) is expressed in carcinomas including breast, lung, colon as well as in metastatic prostate. In 10% of breast cancer patients this gene is amplified, and in 44% the protein is overexpressed.

The gene KCNK9 (potassium two pore domain channel subfamily K member 9) encodes a tandem pore domain potassium channel that dimerizes to form a K+-selective pore. It is highly sensitive to change in pH and is involved in background leak potassium currents found in many cell types. The channel increases the hypoxia tolerance of malignantly transformed cells and facilitates its proliferation and/or survival. It may serve as a potential target for cancer therapies. Mutations in this gene can cause defects in the neuronal migration and have been associated with Birk Barel mental retardation dysmorphism syndrome. This oncogenic potassium channel is found to be expressed in epithelial ovarian cancer and patients with increased expression of this protein have better survival rates.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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