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K3019 Sigma-Aldrich

PKAcα, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: MGC48865

  •  NACRES NA.32

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
Quality Level   200
recombinant   expressed in baculovirus infected Sf9 cells
product line   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
specific activity   1626-2201 nmol/min·mg
mol wt   ~69 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRKACA(5566)

Description

Biochem/physiol Actions

Protein kinase A (PKA) is a mediator of cAMP (cyclic adenosine monophosphate) and contains two regulatory and two catalytic subunits. Mutations in PRKACA (protein kinase cAMP-activated catalytic subunit α) are associated with Cushing′s syndrome. PRKACA encodes two splice variants, Cα1 and Cα2. Cα1 is widely expressed and Cα2 is restricted to the sperm cells.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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