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  • K4261 - Monoclonal Anti-KSR (Kinase Suppressor of Ras) antibody produced in rat

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K4261 Sigma-Aldrich

Monoclonal Anti-KSR (Kinase Suppressor of Ras) antibody produced in rat

~2 mg/mL, clone C3H7D2, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, KL-KY,
conjugate   unconjugated
clone   C3H7D2, monoclonal
biological source   rat
application(s)   immunocytochemistry: suitable using methanol-acetone fixation
  microarray: suitable
  western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing mouse KSR
species reactivity   mouse
mol wt   antigen ~100 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
Quality Level   200
concentration   ~2 mg/mL
UniProt accession no.   Q61097
Gene Information   mouse ... Ksr1(16706)

Description

General description

Kinase Suppressor of Ras (KSR) is a positive effector of Ras signalling pathway. Initially discovered in Drosophila and C. elegans, the KSR family of proteins is structurally similar but distinct from the Raf Family. In response to Ras activation, KSR translocates from the cytoplasm to the plasma membrane and may act as a scaffolding protein to assemble a MAPK signalling complex. KSR interacts with Raf-1, MEK1/2 and ERK1/2 and this interaction is required for a signalling cascade downstream of Ras. Thus KSR plays an important role in survival, proliferation, differentiation, growth and transformation of cells. KSR mediates Ras-dependent signals that are required for neuronal differentiation and functioning of central nervous system
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).

Immunogen

recombinant KSR fragment (amino acids 118-248) from mouse.

Application

Anti-KSR is suitable for immunoblotting at a working concentration of 0.5-1.0 μg/mL using human 293T cells expressing mouse KSR. The antibody may be used for immunocytochemistry and protein microarray applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Safety & Documentation

Safety Information

WGK Germany 
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Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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