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KEM0033 Sigma-Aldrich

T4 DNA Polymerase

Ultra-pure enzyme for nucleic acid modifications

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Properties

grade   for molecular biology
assay   >99% (SDS-PAGE)
form   buffered aqueous solution
specific activity   5,555 U/mg
concentration   3,000 U/mL
shipped in   dry ice
storage temp.   −20°C

Description

General description

T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′ → 3′ direction. This enzyme exhibits a powerful 3′ → 5′ exonuclease activity, while lacking any inherent 5′ → 3′ exonuclease or strand displacement functions.

Application

Suitable for DNA end-repair by fill-in of 5’ overhang and removal of 3’ overhang.

Features and Benefits

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination

Components

Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 6.5 @ 25° C.

Other Notes

Source of protein: Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.

Unit size: 2,000 U

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

DNA Polymerases synthesize DNA from nucleotides.  DNA polymerase is required for DNA replication, but also essential for many other activities in the cell, including genetic recombination, DNA repair...
Keywords: Amplification, Cloning, DNA replication, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Purification, Recombination, Sequencing, Transcription, Whole genome amplification

Protocols

Cloning Protocol for the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

Related Content

Synthetic Biology Resources

We are using our stregnths to help build the field of Synthetic Biology. Below is a collection of links to our favorite resources. In addtion to these resources, we also offer thousands of products f...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Peer-Reviewed Papers
15

References

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