KEM0033 Sigma-Aldrich

T4 DNA Polymerase

Ultra-pure enzyme for nucleic acid modifications



grade   for molecular biology
assay   >99% (SDS-PAGE)
form   buffered aqueous solution
specific activity   5,555 U/mg
concentration   3,000 U/mL
shipped in   dry ice
storage temp.   −20°C


General description

T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′ → 3′ direction. This enzyme exhibits a powerful 3′ → 5′ exonuclease activity, while lacking any inherent 5′ → 3′ exonuclease or strand displacement functions.


Suitable for DNA end-repair by fill-in of 5’ overhang and removal of 3’ overhang.

Features and Benefits

• Ultra-purification process for ultimate enzyme performance
• Highest quality specifications for ultimate product consistency
• Undetectable DNA and nuclease contamination


Supplied with:KEM0042B (10X Blue Buffer)

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37° C.

Physical form

Supplied in 100 mM KPO4, 1.0 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 6.5 @ 25° C.

Other Notes

Source of protein: Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.

Unit size: 2,000 U

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

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Cloning Protocol for the Gene-of-Interest into a Plasmid Vector

Genetic engineering is used in thousands of laboratories around the world. Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. C...
Keywords: Antibiotics, Buffers, Cloning, Degradations, Dialysis, Digestions, Gene expression, Genetic, Genetics, Individual protein Immunoprecipitation, Molecular biology, Nucleic acid annealing, Peptide synthesis, Phosphorylations, Polymerase chain reaction, Precipitation, Purification, Sequencing, Substitutions, transformation

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Peer-Reviewed Papers


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