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L1006 Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

Synonym: (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Biology,
type   Type XVII
form   buffered aqueous glycerol solution
cation traces   NH4+: ≤10 μg/mg protein
foreign activity   glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%
  pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
storage temp.   2-8°C
Gene Information   cow ... LDHA(281274), LDHB(281275)

Description

Analysis Note

Protein determined by biuret

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.

Packaging

12500 units in glass bottle

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA).

General description

L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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