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L1168 Sigma-Aldrich

Anti-phospho-5-Lipoxygenase (pSer523) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Lipids in Cell Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  immunoprecipitation (IP): suitable
  indirect immunofluorescence: suitable
  western blot: 1:1,000
species reactivity   human, rat
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P09917
Gene Information   human ... ALOX5(240)
rat ... Alox5(25290)

Description

General description

The gene ALOX5 (arachidonate 5-lipoxygenase), also referred to as 5-LOX (5-lipoxygenase), encodes a protein belonging to the lipoxygenase family. The gene is mapped to human chromosome 10q11.21. The protein contains a distinct ‘C2-like’ domain at the amino terminal that is involved in Ca2+-dependent membrane binding and a large catalytic domain containing the non-heme catalytic iron. It is highly unstable with short-lived activity.

Immunogen

phosphopeptide corresponding to amino acid residues surrounding the pSer523 of human 5-lipoxgenase (5-LO).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physical form

Solution in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The gene ALOX5 (arachidonate 5-lipoxygenase) encodes a lipoxygenase that catalyzes the conversion of arachidonic acid (AA) and other fatty acids to bioactive compounds, such as 5(S)-hydroxyeicosatetraenoic acid (5-HETE), leukotriene B4 (LTB4), and other leukotrienes. These leukotrienes and lipoxins function as mediators of inflammatory response. ALOX5 has been implicated in tumorigenesis and may serve as a potential therapeutic target and biomarker.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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