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L1754 Sigma-Aldrich

Lipase from Candida rugosa

Type VII, ≥700 unit/mg solid

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
Quality Level   200
type   Type VII
form   lyophilized
specific activity   ≥700 unit/mg solid
storage temp.   2-8°C


General description

Lipases are enzymes and biocatalysts that catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. Lipases are useful for biotransformation reactions. Candida rugosa produces multiple lipase isoenzymes.


Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipases can be used in a wide range of solvents, in both aqueous and non-aqueous media, and thus have found use in applications like organic synthesis.


5, 10, 25, 100 g in poly bottle

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the stability ofProduct L1754, Lipase from Candida rugosa solutions, and how should they be stored?
Solutions in 50% glycerol may be stored at -20°C up to six months. Avoid repeated freeze/thaw cycles. It is best to store aliquots in working volumes.
What is the solubility of Product L1754, Lipase from Candida rugosa?
This product is soluble in water at 1 mg/mL.
What is the difference between Candida rugosa and Candida cylindracea?
These organisms are identical strains. C. rugosa was assigned to what was previously named C. cylindracea by the ATCC.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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