• USA Home
  • L1882 - Lectin from Triticum vulgaris (wheat)

EMAIL THIS PAGE TO A FRIEND
L1882 Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

Synonym: WGA, Wheat germ agglutinin

Purchase

Properties

Related Categories Triticum vulgaris (Wheat), Affinity Chromatography, Carbohydrate Binding Matrices, Glycan Recognizing Proteins, Glycobiology,
form   saline suspension
extent of labeling   5-10 mg per mL
matrix   cross-linked 4% beaded agarose
matrix activation   cyanogen bromide
capacity   ≥0.1 mg/mL binding capacity (ovomucoid)
conjugate   agarose conjugate
storage temp.   2-8°C

Description

Packaging

1, 5 mL in glass bottle

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Physical form

Suspension in 1.0 M NaCl containing 15 Mm Sodium azide.

Preparation Note

Conjugates are prepared from affinity purified lectin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Please Enter a Lot Number
Protocols & Articles

Protocols

Affinity Chromatography Troubleshooting

This section focuses on practical problems that may occur when running a chromatography column. The diagrams below give an indication of how a chromatogram may deviate from the ideal during affnity p...
Keywords: Adsorption, Affinity chromatography, Buffers, Chromatography, Detergents, Digestions, Fractionation, Ligands, PAGE, Precipitation, Purification, Sample preparations, Separation, Solvents

Column Packing and Preparation for Affinity Chromatography

Appendix 3,  Extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Antimicrobials, Buffers, Chromatography, Separation

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (ml/min) and Vice Versa

Appendix 4 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Affinity chromatography, Chromatography

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

Wheat germ lectin can be used for group specific affinity purification of glycoproteins and polysaccharides. This lectin binds N-acetylglucosamine residues and reacts strongly with the chitobiose core o...
Keywords: Absorption, Affinity chromatography, Chromatography, Detergents, Ligands

Sample Preparation for Affinity Chromatography

Appendix 1 extracted from Affinity Chromatography Principles and Methods, GE Healthcare, 2007
Keywords: Adsorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Filtration, Nucleic acid denaturation, PAGE, Precipitation, Sample preparations

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?