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L2130 Sigma-Aldrich

Lactoperoxidase from bovine milk

ammonium sulfate suspension, ≥50 units/mg protein

Synonym: Donor:hydrogen peroxide oxidoreductase, Peroxidase

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Properties

form   ammonium sulfate suspension
specific activity   ≥50 units/mg protein
absorbance ratio   A412/280 nm 0.60-0.85
storage temp.   2-8°C
Gene Information   cow ... LPO(280844)

Description

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 pH approx. 7.0

Analysis Note

Protein determined by Lowry method.

Other Notes

Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

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