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  • L2295 - Anti-LRP1 (N-terminal) antibody produced in rabbit

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L2295 Sigma-Aldrich

Anti-LRP1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Alpha-2-Macroglobulin receptor, Anti-A2MR, Anti-APOER, Anti-APR, Anti-Apolipoprotein E receptor, Anti-CD91, Anti-LRP, Anti-Low density lipoprotein receptor-related protein 1

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Alzheimer′s Disease, Antibodies for Neurobiology, Antibodies for Neurodegenerative Diseases Research,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 2-5 μg/mL using rat NRK cells
  western blot: 1-2 μg/mL using whole extract of human U87 cells
  western blot: 2-4 μg/mL using whole extract of mouse C2C12 cells
species reactivity   human, mouse, rat
mol wt   antigen ~500 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q07954
Gene Information   human ... LRP1(4035)
mouse ... Lrp1(16971)
rat ... Lrp1(299858)

Description

General description

LDL receptor-related protein 1 (LRP1), also known as 2-macroglobulin receptor, is a large endocytic receptor, widely expressed on the surface and intracellular membranes of various cells. LRP1 is encoded by the gene mapped to human chromosome 12q13.3. The encoded protein is a member of the LDL receptor family. LRP1 is synthesized in the endoplasmic reticulum as a single-chain 600 kDa type I transmembrane glycosylated precursor that is cleaved subsequently in the Golgi complex into two subunits of 515 kDa and 85 kDa. The 515 kDa N-terminal subunit remains attached to the membrane through noncovalent association with the smaller 85 kDa C-terminal subunit, which contains the transmembrane and cytoplasmic domains.

Specificity

Anti-LRP1 (N-terminal) recognizes human, mouse, and rat LRP1 (not tested in other species).

Application

Anti-LRP1 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

LRP1 expression is essential for early embryonic development. LRP1 facilitates various biological processes including lipoprotein metabolism, proteinase regulation, lysosomal enzymes activation and entry of viruses and toxins into the cells. In addition, It also regulates cell signaling pathways, cell migration, and neurodevelopment. LRP1 has an ability to bind at least 30 different ligands such as lipoproteins, proteinases, proteinase-inhibitor complexes, extra cellular matrix (ECM) proteins and various intracellular proteins. It also binds to bacterial toxins and viruses.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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