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L2625 Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

Type III, ammonium sulfate suspension, ≥500 units/mg protein

Synonym: (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD, Lactate

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Description

General description

Lactic dehydrogenase (LDH) catalyzes the conversion of lactate to pyruvate in anaerobic glycolysis. It exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality.

Application

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay.

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Crystalline suspension in 2.1 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

Protocols

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

To standardize a procedure for the assay of L-Lactic Dehydrogenase for all sources from heart muscle.
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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