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L3001 Sigma-Aldrich

Lipase from wheat germ

Type I, lyophilized powder, 5-15 units/mg protein

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
type   Type I
form   lyophilized powder
composition   Protein, ≥70%
storage temp.   2-8°C
Gene Information   wheat ... lipase(543006)

Description

General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:
• to prepare enzyme solution for the biodegradability test
• to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
• to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Packaging

1, 5 g in poly bottle

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Quality

Contains acid phosphatase.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TO9776500

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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