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L3126 Sigma-Aldrich

Lipase from porcine pancreas

Type II, 100-650 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

Synonym: PPL, Pancreatic lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
Quality Level   200
type   Type II
form   lyophilized (cake)
specific activity   100-650 units/mg protein (using olive oil (30 min incubation))
  30-90 units/mg protein (using triacetin)
composition   protein, ≥20%
storage temp.   2-8°C

Description

Application

Lipase from porcine pancreas has been used:
• for the analysis of fatty acid at the 2-position of triglycerides.
• to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
• as a component of dissolution media to study the dissolution profiles of theophylline.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Packaging

25, 100, 500 g in poly bottle

Biochem/physiol Actions

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Quality

Crude (Steapsin). Contains amylase and protease activity.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TO9776500
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

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Peer-Reviewed Papers
15

References

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