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  • L3150 - Monoclonal Anti-LEF-1 (Transactivation Domain 236-242) antibody produced in mouse

L3150 Sigma

Monoclonal Anti-LEF-1 (Transactivation Domain 236-242) antibody produced in mouse

~1 mg/mL, clone 3A12, purified immunoglobulin



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood, Antibodies to Common Transcription Factors,
species reactivity   human
application(s)   western blot: 1-5 μg/mL
clone   3A12, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
isotype   IgG1
mol wt   antigen mol wt 55-57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LEF1(51176)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to amino acids 236-242 (DTSMSRF) of human LEF-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.5, containing 0.08% sodium azide.

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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