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  • L3276 - Monoclonal Anti-LEF-1 antibody produced in mouse

L3276 Sigma

Monoclonal Anti-LEF-1 antibody produced in mouse

~1 mg/mL, clone 1C3, purified immunoglobulin

Synonym: Anti-Lymphoid Enhancer Factor-1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Immune Cell Signaling and Blood,
species reactivity   human
application(s)   western blot: 1-5 μg/mL using whole extracts of human Jurkat cells or transiently expressed human LEF-1 in whole cell lysates from Cos-1 cells
clone   1C3, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
isotype   IgG1
mol wt   antigen mol wt 55-57 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... LEF1(51176)
biological source   mouse
conjugate   unconjugated



recombinant full length LEF-1.

Physical form

Solution in 0.01 M phosphate buffered saline containing 0.08% sodium azide.

Preparation Note

Purified from cell culture supernatnant.

Price and Availability

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Certificate of Analysis

Certificate of Origin

Safety Information

WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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