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L3408 Sigma-Aldrich

Transforming Growth Factor-β1 Latency Associated Peptide human

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, cell culture tested

Synonym: LAP, TGF-β1

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Properties

Related Categories Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Cancer Research, Cell Biology,
recombinant   expressed in Sf21 cells
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
mol wt   protein mol wt 70 kDa
impurities   endotoxin, tested
suitability   cell culture tested
storage temp.   −20°C
Gene Information   human ... TGFB1(7040)

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

The biological activity is measured by its ability to inhibit TGF-β1 activity on mouse HT-2 cells.

Physical form

Lyophilized from 0.2 μm filtered phosphate buffered saline containing 1.25 mg bovine serum albumin.

Application

Latency Associated Peptide (LAP) may be used to inhibit transforming growth factor-β 1 activity in various biological systems.

Biochem/physiol Actions

TGF-β1 is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

General description

TGF-β is released from degranulating platelets and secreted from nearly all cells in a biologically inactive complex which is unable to bind to cellular receptors and is not recognized by antibodies to TGF-β. The peptide can be activated by acidification, alkalinization, or action of chaotropic agents in vitro. The complex consists of TGF-β associated non-covalently with a protein designated as the latency associated peptide (LAP). TGF-β and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. The recombinant human LAP is produced from a DNA sequence corresponding to the 278 amino acid residues of pre-pro-TGF-β1 terminating prior to the mature TGF-β1. LAP contains a Cys33 to Ser33 substitution. LAP contains 249 amino acids, generated after cleavage of a 29 amino acid residue signal peptide. LAP is a glycoslyated, disulfide linked homodimer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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