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L3522 Sigma-Aldrich

LB Broth (Miller)

Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Synonym: Luria Broth, Miller’s LB Broth

  •  NACRES NA.85

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Description

General description

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

LB Broth (Miller) has been used as a culture medium for E coli, Pseudomonas putida KT2440, Vibrio parahaemolyticus, and soil samples for the isolation of Bacillus spp.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Features and Benefits

Miller’s LB powder provides:
• Easy scale-up using larger package sizes
• A budget-friendly alternative to liquid
• Standard formulation

Preparation Note

1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.

Reconstitution

Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the differences among the Luria, Lennox and Miller LB formulations?
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).For some applications, even lower salt is required (Luria's low salt version).
What is the difference among the LB - Miller products?
LB - Miller is available in many types to suit your needs.The different product formats include powder and liquid form.The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol.Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Will adding magnesium to the culture medium increase cell density?
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Protocols

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneu...
Keywords: Antibiotics, Cloning, Environmental, Gene expression, Genetic, Molecular biology, Peptide synthesis, Polymerase chain reaction, Transfection, transformation

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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