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L3771 Sigma

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Synonym: Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate



Related Categories Alkyl Sulfates, Anionic Detergents, Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers and Reagents,
grade   for molecular biology
description   anionic
product line   BioReagent
assay   ≥98.5% (GC)
mol wt   micellar mol wt 18,000
aggregation number   62
CMC   7-10 mM(20-25°C)
mp   204-207 °C(lit.)
transition temp   cloud point >100 °C
solubility   water: soluble
anion traces   chloride (Cl-): ≤500 ppm
  phosphate (PO43-): ≤10 ppm
cation traces   heavy metals (as Pb): ≤10 ppm
absorption   A3%/260 ≤0.1
  A3%/280 ≤0.1
HLB   40
suitability   suitable for electrophoresis
  suitable for electrophoresis
foreign activity   DNase, RNase, none detected


Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.


Anionic detergent

Sodium dodecyl sulfate has been used:
• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting


1 kg in poly bottle

25, 100, 500 g in poly bottle

General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Price and Availability

LC-MS Grade Solvents and Reagents

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Safety & Documentation

Safety Information

Signal word 
UN1325 - class 4.1 - PG 3 - Flammable solids, organic, n.o.s., HI: all
WGK Germany 
Flash Point(F) 
338 °F
Flash Point(C) 
170 °C
Protocols & Articles


Detergent Properties and Applications

The key to detergent function is an amphipathic structure. All detergents are characterized as containing a hydrophilic “head” region and a hydrophobic “tail” region (see Figure 1).
Vicki Caligur
BioFiles 2008, 3.3, 14.
Keywords: Adsorption, Biochemistry, Cell disruption, Chromatography, Crystallization, Detergents, Diagnostic, Dialysis, Electrophoresis, Enzymology, Filtration, Mass spectrometry, Normal-phase chromatography, Nucleic acid denaturation, Precipitation, Purification, Separation, Size-exclusion chromatography

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation


Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

Peer-Reviewed Papers


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