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L3771 Sigma-Aldrich

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Synonym: Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate

  • CAS Number 151-21-3

  • Linear Formula CH3(CH2)11OSO3Na

  • Molecular Weight 288.38

  •  Beilstein/REAXYS Number 3599286

  •  EC Number 205-788-1

  •  MDL number MFCD00036175

  •  eCl@ss 39093306

  •  PubChem Substance ID 24896351

  •  NACRES NB.22



Related Categories Alkyl Sulfates, Anionic Detergents, Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers and Reagents,
Quality Level   200
grade   for molecular biology
description   anionic
product line   BioReagent
assay   ≥98.5% (GC)
mol wt   micellar 18,000
aggregation number   62
application(s)   electrophoresis: suitable
CMC   7-10 mM (20-25°C)
mp   204-207 °C (lit.)
transition temp   cloud point >100 °C
solubility   water: soluble
anion traces   chloride (Cl-): ≤500 ppm
  phosphate (PO43-): ≤10 ppm
cation traces   heavy metals (as Pb): ≤10 ppm
absorption   ≤0.1 at 260 at 3%
  ≤0.1 at 280 at 3%
HLB   40
suitability   suitable for electrophoresis
  suitable for electrophoresis
foreign activity   DNase, RNase, none detected
storage temp.   room temp
SMILES string   [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
InChI   1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1


General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.


Sodium dodecyl sulfate has been used:
• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.

Anionic detergent


1 kg in poly bottle

25, 100, 500 g in poly bottle

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Safety & Documentation

Safety Information

Signal word 
Target organs 
Respiratory system
UN1325 - class 4.1 - PG 3 - Flammable solids, organic, n.o.s., HI: all
WGK Germany 
Flash Point(F) 
338.0 °F
Flash Point(C) 
170 °C


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation


Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the ...
Keywords: Alkylations, Cell disruption, Deaminations, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, PAGE, Polymerization reactions, Sample preparations, Separation, Titrations

Protein Extraction and Cell Lysis Protocol

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

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