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L3771 Sigma

Sodium dodecyl sulfate

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Synonym: Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate

  • CAS Number 151-21-3

  • Linear Formula CH3(CH2)11OSO3Na

  • Molecular Weight 288.38

  •  Beilstein Registry Number 3599286

  •  EC Number 205-788-1

  •  MDL number MFCD00036175

  •  eCl@ss 39093306

  •  PubChem Substance ID 24896351

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Properties

Related Categories Alkyl Sulfates, Anionic Detergents, Antigen-Vaccine Preparation, Biochemicals and Reagents, Buffers and Reagents,
grade   for molecular biology
description   anionic
InChI Key   DBMJMQXJHONAFJ-UHFFFAOYSA-M
product line   BioReagent
assay   ≥98.5% (GC)
mol wt   micellar mol wt 18,000
aggregation number   62
CMC   7-10 mM(20-25°C)
mp   204-207 °C (lit.)
transition temp   cloud point >100 °C
solubility   water: soluble
anion traces   chloride (Cl-): ≤500 ppm
  phosphate (PO43-): ≤10 ppm
cation traces   heavy metals (as Pb): ≤10 ppm
absorption   A3%/260 ≤0.1
  A3%/280 ≤0.1
HLB   40
suitability   suitable for electrophoresis
  suitable for electrophoresis
foreign activity   DNase, RNase, none detected

Description

Analysis Note

Tested for use in denatured polyacrylamide gel electrophoresis.

Application

Anionic detergent

Sodium dodecyl sulfate has been used:
• in chromatin immunoprecipitation
• in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
• as a component of radioimmunoprecipitation assay buffer for immunoblotting

Packaging

1 kg in poly bottle

25, 100, 500 g in poly bottle

General description

Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN1325 - class 4.1 - PG 3 - Flammable solids, organic, n.o.s., HI: all
WGK Germany 
2
RTECS 
WT1050000
Flash Point(F) 
338 °F
Flash Point(C) 
170 °C

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Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Sample Preparation for Western Blotting: Cell Lysis and Protein Extraction

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Stripping and Reprobing Western Blotting Membranes

Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane...
Keywords: Buffers, Detergents, Immobilization, PAGE, Western blot

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is s...
Keywords: AGE, Absorption, Biochemistry, Cancer, Cell disruption, Centrifugation, Chromatography, Cloning, Degradations, Electrophoresis, Eliminations, Gel electrophoresis, Gene expression, Genetics, Homogenization, Molecular biology, Northern blot, Polymerase chain reaction, Precipitation, Separation

Peer-Reviewed Papers
15

References

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