• USA Home
  • L4025 - Lyticase from Arthrobacter luteus

L4025 Sigma

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid



Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer


25000, 50000, 100000, 250000, 1000000 units in poly bottle

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.


Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Price and Availability

Expore Enzymes, Proteins & Cofactors

Biomedical Applications

Celebrate Pi Day!
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate. The turbidimetric rate determination [Absorbance at 800 nm (A800), Light path = 1 cm] is ba...
Keywords: Enzyme activity, Sonication

Related Content

Enzyme Explorer Key Resources: Lysing Enzymes

Cell Lysis Enzymes – How to tear down the Wall Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your...
Keywords: Amplification, Biochemistry, Cell culture, Cell disruption, Culture media, Digestions, Enzymology, Metabolites, Molecular biology, PAGE, Purification, Sequencing

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?