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L4402 Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

Synonym: Glycyl-glycine Endopeptidase

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Description

General description

Chemical structure: peptide

Package size based on protein content

Packaging

0.5, 2, 5 mg in glass bottle

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Preparation Note

Affinity purified

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
OL5985000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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