L4777 Sigma-Aldrich

Lipase acrylic resin

Green Alternative

≥5,000 U/g, recombinant, expressed in Aspergillus niger

Synonym: Novozyme 435



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,
recombinant   expressed in Aspergillus niger
greener alternative product characteristics   Waste Prevention: Greener alternative product characteristics
Learn more about the Principles of Green Chemistry.
matrix   macroporous acrylic resin
Featured Industry   Diagnostic Assay Manufacturing
greener alternative category   Enabling
storage temp.   2-8°C


General description

Lipase from produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in biodiesel research. For more information see the article in biofiles.


3, 10 g in poly bottle


Immobilized preparation of a thermostable lipase, particularly useful in the synthesis of esters and amides, and has a broad substrate specificity.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What solvents are most compatible with Product L4777, Lipase acrylic resin?
This resin works well in solvent-free reactions, as well as in inert water immiscible solvents such as petroleum ether, n-hexane and n-heptane. If the reaction being catalyzed is hydrolysis of an ester, addition of water to the reaction mix is recommended. For other reactions water is likely not required, and in some cases would actually be detrimental.
What is the substrate specificity of Product L4777, Lipase acrylic resin?
Lipase acrylic resin has a broad substrate specificity. It promotes reactions between a wide range of primary and secondary alcohols and carboxylic acids.
What are the components of Product L4777, Lipase acrylic resin, and what are the optimal recommended conditions?
This product consists of a thermostable lipase enzyme immobilized on a macroporous acrylic bead resin. The bead-shaped particles have a diameter in the range of 0.3-0.9 mm. The resin will be compatible with stearyl alcohol at temperatures of 40-60°C, which is the normal operating temperature of the enzyme. This immobilized enzyme can be used in hexane with no adverse effects.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles


HPLC Analysis of Various Polymers on TSKgel® SuperµLtiporeHZ-H

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Various Polymers on TSKgel® SuperµLtiporeHZ-H
Keywords: Chromatography, High performance liquid chromatography

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?