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L5158 Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

Synonym: LIF




Leukemia Inhibitory Factor from mouse has been used:
• as a component of enriched co-culture media
• as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
• as a component in maintenance medium for maintaining embryonic stem cells
• to treat ovaries in ovarian culture

Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Analysis Note

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the sequence of the protein Leukemia Inhibitory Factor, Product L5158?
The partial sequence is reported on the SwissProt website under the accession number P09056. The recombinant has 1 more amino acid but the supplier has kept details as proprietary.
How is this recombinant protein, Leukemia Inhibitory Factor, Product L5158,prepared?
The protein is expressed in E. coli as a GST-tagged fusion protein.After purification, the tag is removed by thrombin treatment to product the final protein sequence.
IsLeukemia Inhibitory Factor, Product L5158, the same as the LIF from Chemicon?
Chemicon holds the patent for manufacture of mouse recombinant LIF.The product from Sigma-Aldrich is identical to that from Chemicon.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Related Content

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) belongs to the interleukin-6 cytokine family. LIF binds to a heterodimeric receptor consisting of the low-affinity LIF receptor and gp130, with downstream signals bei...
Keywords: Cell culture, Culture media, Growth factors, Transcription

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