L6250 Sigma

Lithocholic acid


Synonym: 3α-Hydroxy-5β-cholan-24-oic acid, 3α-Hydroxy-5β-cholanic acid, 5β-Cholan-24-oic acid-3α-ol

  • CAS Number 434-13-9

  • Empirical Formula (Hill Notation) C24H40O3

  • Molecular Weight 376.57

  •  Beilstein Registry Number 3217757

  •  EC Number 207-099-1

  •  MDL number MFCD00003682

  •  PubChem Substance ID 24896429



Related Categories Bile acids and Derivatives, Biochemicals and Reagents, Lipids, Sterol Lipids
assay   ≥95%
mp   183-188 °C(lit.)
Gene Information   human ... POLA1(5422), TOP2A(7153)
rat ... Polb(29240)


Biochem/physiol Actions

Bile Acid

General description

Lithocholic acid is an oligo(ethylene glycol) derivative of bile acid.


10, 25 g in poly bottle


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Lithocholic acid has been used in a study to assess cholestasis and its action on several organs and tissues in rats. It has also been used in a study to investigate the regulation of hepatic phospholipid and bile acid homeostasis through SMAD3 activation by TGFβ.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. L6250.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Webinar - Hydrogels
Protocols & Articles


Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Fast and Robust HPLC Separation of Bile Acids and Conjugates with Ascentis Express C18

Bile acids are increasingly recognized as playing an important signaling role in the control of immune response and energy metabolism. Recently, there has been interest in measuring bile acid concent...
Clare Glicksman, Michael Wright, Dave Bell, and Anders Fridström
Reporter US, Volume 33.1
Keywords: Bacterial conjugations, Clinical, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolism, Separation, Thin layer chromatography

HPLC Analysis of Bile Acids on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bile Acids on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

TLC Analysis Report of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

From our library of Articles, Sigma-Aldrich presents TLC Analysis Report of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s
Keywords: Chromatography, Thin layer chromatography

Peer-Reviewed Papers


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