• USA Home
  • L6274 - Laminin from human placenta

EMAIL THIS PAGE TO A FRIEND
L6274 Sigma-Aldrich

Laminin from human placenta

liquid, BioReagent, suitable for cell culture

Purchase

Description

Components

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.

Biochem/physiol Actions

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Caution

It is recommended to store this product at -70°C, where it will remain stable for two years.

Preparation Note

This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do you prepare Product L6274, Laminin from human placenta?
To prepare a laminin solution, we recommend that the L6274 be thawed at 2-8°C to avoid the formation of a gel. This can be further diluted in a balanced salt solution. If the product has been thawed at room temperature and ends up as a gel, it cannot be reactivated for use.
How long can cover slips coated with Product L6274, Laminin from human placenta, be stored?
Laminin coatings, such as laminin coated cover slips last about one month at 2-8°C. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.
Is Product L6274, Laminin from human placenta,laminin 1 or 2?
Fifteen laminin trimers have been identified. We do not determine what type of laminin this is. Typically laminin from placenta is considered laminin-2/4 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane is laminin-1.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Cancer Stem Cells: New Targets for Cancer Therapy

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

Laminin - BioFiles 2008, 3.8, 11.

Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth (PHD) fragment.1 Laminin chains are designated A (molecular weight 400kDa), B1 (molecular weight...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Adhesion, Biofiles, Cell culture

Laminin Cell Attachment Protocol

Q1) Can I coat laminin on top of poly-L-lysine for better adherence? A1) When coating coverslips or slides for use with neural stem cells (neurospheres) the following procedure is used. Precoat the c...
George Sitterley
BioFiles 2008, 3.8, 11.
Keywords: Biofiles, PAGE, SDS PAGE

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?