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L6544 Sigma-Aldrich

LuminoCt® SYBR® Green qPCR ReadyMix

For fast SYBR Green quantitative PCR

Synonym: LuminoCt®

Purchase

Properties

Related Categories Fast QPCR, LuminoCt qPCR ReadyMix, Molecular Biology, Nucleic Acid Amplification, PCR/Amplification,
form   liquid
feature   hotstart
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C

Description

General description

LuminoCt SYBR Green qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Application

LuminoCt® SYBR® Green qPCR ReadyMix has been used for fast qPCR and quantitative RT-PCR amplifications.

Features and Benefits

• Assay results in as little as 25 minutes
• Unsurpassed accuracy, precision, and sensitivity
• Virtually eliminates the need to optimize assay parameters

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

• At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
• Sigma recommends designing primers and probes with Beacon Designer software
• To order primers and probes from Sigma, visit: www.sigma.com/oligos

Legal Information

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LuminoCt is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

SabreLite is a registered trademark of Pelican Products, Inc.

Kit component only

Description

Product #

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LuminoCt SYBR Green qPCR ReadyMix 2 X    

Standard component

Description

Product #

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100X ROX internal reference dye SDS R4526
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Polymerase Chain Reaction - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Amplification, Cloning, Degradations, Detection methods, Electrophoresis, Evaporation, Gas chromatography, Gel electrophoresis, Indicators, Melting, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Size-exclusion chromatography, Transcription

Protocols

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

PCR Master Mix

Sometimes known as super mix or ready mix, PCR master mix is a batch mixture of PCR reagents at optimal concentrations that can be prepared once and divided among many PCR tubes or 96-well PCR plates...
Keywords: Buffers, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
Keywords: Building blocks, Mass spectrometry, Organic synthesis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Vitamins

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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Need larger quantities for your development, manufacturing or research applications?