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L6669 Sigma-Aldrich

LuminoCt® qPCR ReadyMix

For fast probe-based quantitative PCR

Synonym: LuminoCt® qPCR ReadyMix, LuminoCt®

  •  NACRES NA.55

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Properties

Related Categories Fast QPCR, LuminoCt qPCR ReadyMix, Molecular Biology, Nucleic Acid Amplification, PCR/Amplification,
Quality Level   200
form   liquid
feature   hotstart
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C

Description

Application

LuminoCt® qPCR ReadyMix has been used in qRT-PCR (real-time quantitative reverse transcription polymerase chain reaction) analysis of osteogenic differentiation.

LuminoCt qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers and probes. Inclusion of JumpStart Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes.

Features and Benefits

• Assay results in as little as 25 minutes
• Unsurpassed accuracy, precision, and sensitivity
• Virtually eliminates the need to optimize assay parameters

Packaging

LuminoCt qPCR ReadyMix, Catalog Number L5794, contains optimized concentrations of Tris-HCl, pH 8.3, KCl, dNTPs (dATP, dCTP, dGTP, TTP), stabilizers, MgCl2 and JumpStart Taq DNA Polymerase. Provided as 100, 500 and 2000 reactions (25 μL mix in a 50 μL reaction volume).

100x ROX internal reference dye, Catalog Number R4526. Optional, for use with machines compatible with an internal reference dye, e.g., ABI and Stratagene.

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

• Commercially available 5′ nuclease assays (e.g., TaqMan® Assays) are compatible with LuminoCt ReadyMix.
• At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are <200 bp.
• Sigma recommends designing primers and probes with Beacon Designer software

To order primers and probes from Sigma, visit www.sigma.com/oligos

Legal Information

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LuminoCt is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit component also available separately

Description

Product #

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100X ROX internal reference dye SDS R4526
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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