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L7386 Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Synonym: Glycyl-glycine Endopeptidase

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Antibacterial, Antibiotics, Antibiotics A to Z,
Quality Level   300
biological source   microbial (Staphylococcus staphylotyticus)
form   lyophilized powder
specific activity   ≥500 units/mg protein
mol wt   25 kDa
composition   Protein, 50-70% biuret
packaging   pkg of (sold on basis of mg protein)
solubility   water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow
Featured Industry   Diagnostic Assay Manufacturing
Diagnostic Assay Manufacturing
Mode of action   cell wall synthesis | interferes
antibiotic activity spectrum   Gram-positive bacteria
shipped in   wet ice
storage temp.   −20°C

Description

General description

Chemical structure: peptide

Packaging

1, 5, 15 mg in glass bottle

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
OL5985000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

GenElute Bacterial Genomic DNA Kit Protocol

Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and purify...
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GenElute Bacterial Genomic DNA Kit, Experienced User Protocol

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information.

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Peer-Reviewed Papers
15

References

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