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L7386 Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

Synonym: Glycyl-glycine Endopeptidase



Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Antibacterial, Antibiotics, Antibiotics A to Z,
Quality Level   300
biological source   microbial (Staphylococcus staphylotyticus)
form   lyophilized powder
specific activity   ≥500 units/mg protein
mol wt   25 kDa
composition   Protein, 50-70% biuret
packaging   pkg of (sold on basis of mg protein)
solubility   water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow
Featured Industry   Diagnostic Assay Manufacturing
Diagnostic Assay Manufacturing
Mode of action   cell wall synthesis | interferes
antibiotic activity spectrum   Gram-positive bacteria
shipped in   wet ice
storage temp.   −20°C


General description

Chemical structure: peptide


1, 5, 15 mg in glass bottle

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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