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L7395 Sigma-Aldrich

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

Synonym: Linoleate:oxygen oxidoreductase, Lipoxygenase

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Properties

Related Categories 1.13.x.x Acting on single donors with incorporation of molecular oxygen, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
Quality Level   200
type   Type I-B
form   lyophilized powder
mol wt   108 kDa (two 54 kDa subunits)
shipped in   wet ice
storage temp.   −20°C

Description

Packaging

15000000, 75000000, 150000000 units in poly bottle

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Lipoxidase on TSKgel® Phenyl-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lipoxidase on TSKgel® Phenyl-5PW
Keywords: Chromatography, High performance liquid chromatography

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Peer-Reviewed Papers
15

References

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