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L7917 Sigma-Aldrich

Anti-LKB1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-PJS, Anti-STK11

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, L-LL,
species reactivity   mouse, human, rat
application(s)   microarray: suitable
  western blot: 1:2,000-1:4,000 using a cytosolic fraction (S1) of mouse brain.
  western blot: 1:2,000-1:4,000 using a whole cell extract of the rat fibroblasts Rat-1 cell line and a whole cell extract of the human epidermoid carcinoma A431 cell line.
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~55 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... STK11(6794)
mouse ... Stk11(20869)
rat ... Stk11(314621)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated
UniProt accession no.   Q15831

Description

Immunogen

synthetic peptide corresponding to amino acids 422-436 located at the C-terminus of mouse LKB1, conjugated to KLH. This sequence is highly conserved (90%) in human LKB1.

General description

LKB1 is a serine/threonine kinase that functions as a tumor suppressor. LKB1 mediates several functions such as initiation of axons during neuronal polarization, spermiogenesis and hepatic glucose homeostasis. Mutations in LKB1 have been linked to Peutz-Jeghers syndrome . Anti-LKB1 antibody is specific for mouse, human and rat LKB1 (55 kDa). In immunoblotting, staining of LKB1 is specifically inhibited with the LKB1 immunizing peptide (mouse, amino acids 422-436).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-LKB1 antibody is suitable for use in western blot at 1:2,000-1:4,000 dilutions, using cytosolic fractions (S1) of mouse brain or whole cell extracts of rat fibroblasts (Rat-1 cell line) and human epidermoid carcinoma (A431 cell line). The antibody can also be used for immunofluorescence and microarray studies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Oncogenes and Tumor Suppressors Reprogram Metabolism

Proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication. This requires a reprogramming of the metabolic pathways to ensure nutrients s...
Keywords: Aerobic, Antitumor agents, Apoptosis, Biofiles, Cancer, Citric Acid Cycle, Degradations, Environmental, Events, Gene expression, Glycolysis, Growth factors, Metabolic Pathways, Metabolism, Metabolites, Nucleotide Synthesis, PAGE, Pentose phosphate pathway, Phosphorylations, Support

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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L9168 Monoclonal Anti-LKB1 antibody produced in mouse, ~1 mg/mL, clone Ley37D/G6, purified immunoglobulin, buffered aqueous solution

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