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L8257 Sigma-Aldrich

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥80 units/mg protein

Synonym: Donor:hydrogen peroxide oxidoreductase, Peroxidase

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Properties

Related Categories 1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder (essentially salt-free)
specific activity   ≥80 units/mg protein
absorbance ratio   A412/280 nm 0.88-0.95
storage temp.   −20°C
Gene Information   cow ... LPO(280844)

Description

Biochem/physiol Actions

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Unit Definition

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Analysis Note

Protein determined by Lowry method.

Other Notes

Using 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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