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L8402 Sigma-Aldrich

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase



Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... LYZ(4069)


General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.


Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

Biochem/physiol Actions

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

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Certificate of Origin

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Protocols & Articles


Enzymatic Assay of Lysozyme (EC

This procedure may be used for the enzymatic assay of Lysozyme products. The spectrophotometric rate determination (A450, Light path = 1 cm) is based on the following reaction:

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