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L9031 Sigma-Aldrich

Lipase from Mucor miehei

lyophilized powder, ≥4,000 units/mg solid (using olive oil)

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
Quality Level   100
form   lyophilized powder
specific activity   ≥4,000 units/mg solid (using olive oil)
storage temp.   2-8°C


General description

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.


Lipase from Mucor miehei can be used to drive the synthesis of flavor esters.

Lipase from Mucor miehei has been used in a study to assess the nonthermal effect of microwave irradiation in nonaqueous enzymatic esterification. It has also been used in a study to investigate the hydrolysis of virgin coconut oil using immobilized lipase in a batch reactor.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Mucor miehei lipase hydrolizes triglycerides and catalyzes the synthesis of esters from glycerol and long-chain fatty acids in vitro.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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