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  • L9277 - Anti-Leukemia Inhibitory Factor antibody produced in goat

L9277 Sigma-Aldrich

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-LIF



General description

Leukemia inhibitory factor (LIF) is a pleotrophic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions.
Anti-Leukemia Inhibitory Factor recognizes human leukemia inhibitory factor. It shows no cross-reactivity with recombinant mouse LIF.


The antibody neutralizes the biological activity of recombinant human LIF.


recombinant human LIF expressed in E. coli


Anti-Leukemia Inhibitory Factor antibody may be for immunoblotting a concentration of 1 μg/ml For ELISA a working concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions. For neutralization of LIF in SEK1, melanoma cell line, a concentration of 100 μg/ml was used.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Preparation Note

Purified using protein G

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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