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L9393 Sigma-Aldrich

Anti-Laminin antibody produced in rabbit

Antibody Enhanced Validation

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Laminin Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix, Antibodies to Extracellular Matrix Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:1,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues
  microarray: suitable
species reactivity   human, animal
enhanced validation   independent
Learn more about Antibody Enhanced Validation
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   0.5 mg/mL
Featured Industry   Research Pathology
UniProt accession no.   P25391
Gene Information   human ... LAMA1(284217)
mouse ... Lama1(16772)

Description

General description

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.

Specificity

Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.

Immunogen

Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

Application

Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.

Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the protein concentration of Anti-Laminin antibody, Product L9393?
The protein concentration is Lot/Batch dependent and is located on the Certificate of Analysis.
What is the species reactivity of Anti-Laminin antibody, Product L9393?
This affinity-isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources. This information is located on the product data sheet.
What applications is Anti-Laminin antibody, Product L9393, suitable for?
Rabbit Anti-Laminin may be used in immunohistochemistry, immunofluorescence, and dot blot analysis.This information is located on the product datasheet available on our website.
Can Anti-Laminin antibody, Product L9393, be used in Western blot analysis?
The following reference uses this antibody for Western blot at 1:1000.DiProspero, N.A., et al., Inflammatory Cytokines Interact to Modulate Extracellular Matrix and Astrocytic Support of Neurite Outgrowth. Exp. Neurol., 148, 628-639 (1997).
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Articles

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Peer-Reviewed Papers
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