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L9420 Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Synonym: Luciferase firefly

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Properties

Related Categories Analytical and Industrial Enzymes, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Luciferase, Purified Enzymes More...
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C

Description

General description

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Application

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Unit Definition

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Physical form

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Flash Point(F) 
231.8 °F
Flash Point(C) 
111 °C
Protocols & Articles

Articles

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in ...
Keywords: Catalysis, Cell proliferation, Enzyme activity, Gene expression, Genetic, Genomics, Indicators, Oxidations, Post translational modifications, Titrations, Transfection

TokeOni: Properties and Applications

Bioluminescence imaging (BLI) systems allow for high-sensitive and noninvasive monitoring of cell proliferation, activity of signaling pathways and protein-protein interactions in living tissues.1-3 ...
Takahiro Kuchimaru and Shinae Kizaka-Kondoh
Graduate School of Bioengineering & Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Keywords: Cancer, Cell proliferation, Infrared spectroscopy

Peer-Reviewed Papers
15

References

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