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L9518 Sigma-Aldrich

Lipase from Pseudomonas sp.

Type XIII, lyophilized powder, ≥15 units/mg solid

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
Quality Level   200
type   Type XIII
form   lyophilized powder
specific activity   ≥15 units/mg solid
mol wt   ~134 kDa
composition   Protein, 40-65% biuret
storage temp.   2-8°C

Description

General description

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.

Application

This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37oC by using 2.5 oC~3.0 units of the enzyme per test (3.0ml) at pH around 7.0. Lipase from Pseudomonas sp. has been used in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation.

Packaging

500, 1000 units in poly bottle

Biochem/physiol Actions

Lipase from Pseudomonas was shown to inhibit monocytes chemotaxis in human peripheral blood.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Physical properties

Isoelectric point : 5.95 -/+0.05
Inhibitors : Hg++, Ag+, ionic detergents
Optimum pH : 7.0 - 9.0
Optimum temperature : 45 - 50oC
pH Stability : pH 7.0 - 9.0 (25oC, 20hr)
Thermal stability : below 55oC (pH 7.0, 10min)

Unit Definition

One unit will produce 1.0 μmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin.

Physical form

Lyophilized powder containing Mg+2, sodium cholate, and bovine serum albumin as stabilizers

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Protocols

Assay Procedure for Lipase

One unit causes the formation of one micromole of glycerol (half a micromole of quinoneimine dye) per minute under the conditions described below.
Keywords: Extinction coefficient, Filtration

Enzymatic assay of lipase type XIII from Pseudomonas sp. and Pseudomonas cepacia using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas sp., Product No. L9518 , and Pseudomonas cepacia, using a coupled enzyme system of glycerol kinase and glycerop...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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