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L9518 Sigma-Aldrich

Lipase from Pseudomonas sp.

Type XIII, lyophilized powder, ≥15 units/mg solid

Synonym: Triacylglycerol acylhydrolase, Triacylglycerol lipase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Diagnostic and Analytical Enzymes,
type   Type XIII
form   lyophilized powder
mol wt   ~134 kDa
composition   Protein, 40-65% biuret
storage temp.   2-8°C


General description

Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols.


This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α- glycerophosphate oxidase (G3O-301, G3O-311, G3O-321) and glycerol kinase (GYK-301, GYK-311). Usually, the reaction can be completed in 5 minutes at 37oC by using 2.5 oC~3.0 units of the enzyme per test (3.0ml) at pH around 7.0. Lipase from Pseudomonas sp. has been used in a study to assess enzymatic synthesis of biodiesel from palm oil assisted by microwave irradiation.


500, 1000 units in poly bottle

Biochem/physiol Actions

Lipase from Pseudomonas was shown to inhibit monocytes chemotaxis in human peripheral blood.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Physical properties

Isoelectric point : 5.95 -/+0.05
Inhibitors : Hg++, Ag+, ionic detergents
Optimum pH : 7.0 - 9.0
Optimum temperature : 45 - 50oC
pH Stability : pH 7.0 - 9.0 (25oC, 20hr)
Thermal stability : below 55oC (pH 7.0, 10min)

Unit Definition

One unit will produce 1.0 μmole of glycerol from a triglyceride per min at pH 7.0 at 37 °C in the presence of bovine serum albumin.

Physical form

Lyophilized powder containing Mg+2, sodium cholate, and bovine serum albumin as stabilizers

Analysis Note

Protein determined by biuret.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Assay Procedure for Lipase

One unit causes the formation of one micromole of glycerol (half a micromole of quinoneimine dye) per minute under the conditions described below.
Keywords: Extinction coefficient, Filtration

Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC

To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia, Sigma Product Number L9156, using a coupled enzy...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

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Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers


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