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  • L9544 - Anti-Livin (C-terminal) antibody produced in rabbit

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L9544 Sigma-Aldrich

Anti-Livin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-BIRC7, Anti-Baculoviral IAP repeat-containing 7, Anti-KIAP, Anti-MLIAP, Anti-RNF50

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Suppressors of Apoptosis,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 5-10 μg using lysate of HEK-293T cells expressing human Livin
  western blot: 2.5-5.0 μg/mL using whole extract of HEK-293T cells expressing human Livin
species reactivity   human
mol wt   antigen ~39 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   Q96CA5
Gene Information   human ... BIRC7(79444)

Description

General description

Livin or baculoviral IAP repeat-containing 7 (BIRC7) belongs to the family of the inhibitor of apoptosis proteins (IAP). It contains a single copy of a baculovirus IAP repeat (BIR) as well as a really interesting new gene (RING)-type zinc finger domain. Structurally, livin comprises a three-stranded anti-parallel β-sheet and four α-helices and takes a globular architecture. BIRC7 gene is mapped to human chromosome 20q13.33 and is expressed in α and β isoforms.

Specificity

Anti-Livin (C-terminal) recognizes human Livin/BIRC7.

Application

Anti-Livin (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Livin or baculoviral IAP repeat-containing 7 (BIRC7) inhibitory functionality is mediated by the baculovirus inhibitor of apoptosis proteins (IAP)repeat (BIR) domain. This domain is also essential for interaction with caspases. The really interesting new gene (RING) finger domain elicits E3-ubiquitin ligase activity in the absence of BIR domain. The two isoforms of livin have different antiapoptotic properties, with isoform protecting cells from apoptosis induced by staurosporine and isoform α protecting cells from apoptosis induced by etoposide. An elevated expression of Livin is detected in prostate, breast, colon, head and neck squamous cell carcinoma. Inhibition of livin in nasopharyngeal carcinoma (NPC) leads to improved irradiation sensitivity in NPC cells.

Safety & Documentation

Safety Information

WGK Germany 
1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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L9669 Anti-Livin (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
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