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LIG2 Sigma-Aldrich

QuickLink DNA Ligation Kit

joins blunt-end and sticky-end DNA fragments



Related Categories Cell Biology, Circuit Design and Metabolic Pathway Engineering, Cloning, Directional Cloning System, Modifying Enzymes,
Quality Level   200
grade   for molecular biology
form   liquid
usage    kit sufficient for 50 ligation reactions
shipped in   dry ice
storage temp.   −70°C


General description

Sigma’s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions at room temperature using blunt or sticky ends. This kit replaces previous workflows requiring cooking steps and long incubations. Pre-made buffers allow for fast & easy setup.


QuickLink DNA Ligation Kit has been used for the ligation of pGL2-basic luciferase-reporter plasmid with vascular endothelial growth factor (VEGF) promoter sequence and for the ligation of NLR family caspase activating and recruitment domain (CARD)-containing protein 4 (Nlrc4) promoter with the luciferase plasmid vector pGL4.10-luc2

The QuickLink DNA Ligation Kit is suitable for:
• Joining blunt or cohesive-end fragments of DNA into a cloning vector
• Recircularization of linear DNA
• Formation of concatamers
• dsDNA nick repair

Features and Benefits

• Fast 5 minutes ligation
• High ligation efficiency
• Room temperature reactions – no cooling required
• Perform bacterial transformation with the reaction mixture


Sufficient for 50 reactions:
• 500uL 2X Ligation Buffer A (L9537)
• 100uL 5X Ligation Buffer B (L9662)
• 250 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM DTT


One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

Legal Information

QuickLink is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


QuickLink™ DNA Ligation Kit

Product Description Components Reagents Required But Not Provided Precautions and Disclaimer Storage/Stability Procedure Troubleshooting Guide Materials References
Keywords: AGE, Antibiotics, Cloning, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Polymerase chain reaction, Precipitation, Purification, transformation

Peer-Reviewed Papers


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