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LUC1 Sigma-Aldrich

Luciferase Reporter Gene Detection Kit

  •  NACRES NA.84

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Description

Other Notes

Firefly luciferase is one of the most commonly utilized reporter genes for the study of gene expression. It is an extremely sensitive, rapid, and easy to use reporter gene. The chemiluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. The luciferase substrate contains coenzyme A for increased and sustained luminescence compared to conventional methods. This eliminates the need for automated luminometer injection of substrate and allows analysis by photographic film or scintillation counting. The lysis buffer contains polymyxin B which further enhances the signal and eliminates lysozyme treatment and freeze-thawing of cells. Cell lysis buffer is compatible with β-galactosidase assays.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in ...
Keywords: Catalysis, Cell proliferation, Enzyme activity, Gene expression, Genetic, Genomics, Indicators, Oxidations, Post translational modifications, Titrations, Transfection

Peer-Reviewed Papers
15

References

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