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M0194 Sigma-Aldrich

Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym: Muscarinic Acetylcholine Receptor M3 Antibody - Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit, Muscarinic Acetylcholine Receptor M3 Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:200 using rat brain lysate
species reactivity   mouse, human, rat
form   lyophilized powder
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P20309
Gene Information   human ... CHRM3(1131)
mouse ... Chrm3(12671)
rat ... Chrm3(24260)

Description

General description

Cholinergic receptor muscarinic 3 (CHRM3) gene is located on the human chromosome at 1q43 and encodes for the M3 subtype of muscarinic acetylcholine (ACh) receptors. Muscarinic receptor 3 (M3R) is one of the five muscarinic receptors (M1R-M5R). It is a member of the class A family of G-protein coupled receptors (GPCRs). M3R is localized to the salivary glands, lachrymal glands, trachea and bronchi.

Specificity

Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody recognizes rat, human, mouse Muscarinic Acetylcholine Receptor (M3).

Immunogen

synthetic peptide corresponding to amino acid residues 461-479 of the third intracellular loop of rat muscarinic acetylcholine receptor M3. This sequence is identical in human, mouse, pig, and bovine.

Application

Anti-Muscarinic Acetylcholine Receptor (M3) antibody produced in rabbit has been used in immunohistochemistry and western blotting.

Rabbit polyclonal anti-Muscarinic Acetylcholine Receptor (M3) antibody is used to tag muscarinic acetylcholine receptor (M3) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of muscarinic acetylcholine receptor (M3) in information processing and plastic changes in CNS function.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Muscarinic receptor 3 (M3R) is involved in the contraction of the detrusor muscle. It is also involved in the secretion of saliva and bladder contraction. It participates in several pathological processes of lung disease, such as chronic obstructive pulmonary disease (COPD). Mutations in the CHRM3 gene is associated with congenital bladder malformations related to prune-belly-like syndrome (PBS).

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3288 6.1 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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