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M0404 Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym: MS Basal Medium, Murashige and Skoog (Gamborg Modified) Basal Media

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Properties

Related Categories Culture Media, Salts and Vitamin Mixes, Molecular Biology, Plant Biotechnology, Plant Tissue Culture
Quality Level   200
form   powder
application(s)   cell culture | plant: suitable
Featured Industry   Agriculture
shipped in   ambient
storage temp.   2-8°C

Description

Application

Murashige and Skoog Basal Medium has been used:
• to germinate the seeds for seedling growth and germination studies
• in the suspension culture of Nicotiana plumbaginifolia for transformation
• in the root culture of hairy clone A. artemisiifolia T4

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 1477 5.1 / PGII
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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