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M0449 Sigma

MEKK3, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: MAP3K3, MAPKKK3

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   17-23 nmol/min·mg
mol wt   mol wt ~105 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... MAP3K3(4215)

Description

Biochem/physiol Actions

MEKK3 is a member of the MEKK family of protein kinases that directly regulate SAPK and ERK pathway but not the p38 pathway. MEKK3 modulates SAPK and ERK activity by activating the upstream targets SEK and MEK1/2, respectively. KSR2 can regulate the activity of MEKK3 and can reduce MEKK3-mediated ERK activation. Knockout of the MEKK3 gene in mice leads to disruption of blood vessel development and the structural integrity of the yolk sac. The blockage of angiogenesis in these mice is not a result of decreased VEGF1 expression but rather due to an intrinsic defect in Mekk3 -/- endothelial cells. Thus, MEKK3 is necessary for blood vessel development and may be a possible target for drugs that control angiogenesis.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Expore Enzymes, Proteins & Cofactors

Biomedical Applications
Protocols & Articles
Peer-Reviewed Papers
15

References

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