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M1254 Sigma-Aldrich

MOPS

≥99.5% (titration)

Synonym: 3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid

  • CAS Number 1132-61-2

  • Empirical Formula (Hill Notation) C7H15NO4S

  • Molecular Weight 209.26

  •  Beilstein/REAXYS Number 1106776

  •  EC Number 214-478-5

  •  MDL number MFCD00006183

  •  PubChem Substance ID 24896628

  •  NACRES NA.25

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z, Good’s Buffers,
Quality Level   200
assay   ≥99.5% (titration)
form   crystalline powder
application(s)   electrophoresis: suitable
pH   2.5-4 (25 °C, 209 g/L)
useful pH range   6.5 - 7.9
pKa (25 °C)   7.2
solubility   water: 0.5 g/mL, clear, colorless
λ   33 % in H2O
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   OS(=O)(=O)CCCN1CCOCC1
InChI   1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
InChI key   DVLFYONBTKHTER-UHFFFAOYSA-N

Description

General description

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Application

MOPS has been used as:
• a cell culture additive component in lentiviral particle production
• as a buffering agent in microbial growth medium and nuclei extraction buffer

Packaging

1 kg in poly bottle

25, 100, 250 g in poly bottle

5, 25, 50 kg in poly drum

Other Notes

Easily compare specifications for MOPS products with the MOPS specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
QE9104530
Flash Point(F) 
230.0 °F - closed cup
Flash Point(C) 
110 °C - closed cup

pHast Pack
Protocols & Articles

Articles

UHPLC Analysis NIST mAb Variants on Proteomix SCX-NP5: Comparing Salt and pH Gradients

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis NIST mAb Variants on Proteomix SCX-NP5: Comparing Salt and pH Gradients
Keywords: Chromatography, Ion Exchange, Separation

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Polyacrylamide gel electrophoresis (PAGE) is a foundational technique used to separate proteins and other macromolecules by their electrophoretic mobility. Polyacrylamide gels are formed through the ...
Keywords: Alkylations, Cell disruption, Deaminations, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, PAGE, Polymerization reactions, Sample preparations, Separation, Titrations

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Peer-Reviewed Papers
15

References

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