• USA Home
  • M1302 - M-MLV Reverse Transcriptase

M1302 Sigma-Aldrich

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

Synonym: Moloney Murine Leukemia Virus Reverse Transcriptase



General description

M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid.


M-MLV Reverse Transcriptase has been used:
•  to synthesize cDNA
•  in quantitative realtime-polymerase chain reaction (RT-qPCR)
•  in reverse transcription

M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand. M-MLV is used for the preparation of cDNA libraries or for first strand cDNA synthesis for use in RT-PCR reactions.

Features and Benefits

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.


Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Unit Definition

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

Reverse Transcription - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Degradations, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

RT-PCR | Reverse Transcriptase PCR

RT-PCR, or reverse transcriptase PCR, is a variation of standard PCR technique that involves the amplification of specific mRNA obtained from very small samples. It eliminates the need for the tediou...
Keywords: Amplification, Cloning, Diagnostic, Microbiology, Molecular biology, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription, Whole genome amplification

Peer-Reviewed Papers


Related Products


Product #


Add to Cart

N8878 Nalidixic acid, ≥98%
N4382 Nalidixic acid sodium salt, powder

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?