• USA Home
  • M1814 - MKK7 α1, active human

EMAIL THIS PAGE TO A FRIEND
M1814 Sigma

MKK7 α1, active human

>70% (by Coomassie blue staining following SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution

Synonym: MAP kinase kinase 7 α1

Purchase

Properties

recombinant   expressed in E. coli
assay   >70% (by Coomassie blue staining following SDS-PAGE)
form   buffered aqueous solution
mol wt   mol wt 65 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... MAP2K7(5609)

Description

Application

May be used for protein phosphorylation assay.

Other Notes

GST fusion protein encoding the last 333 residues of human MKK7α1

Preparation Note

Affinity purified using glutathione-agarose and activated with MEK kinase.

Unit Definition

1 unit of active MKK7α1 will transfer 1 nmole of phosphate to ATF2 per minute at 30 °C.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 270 mM sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 0.1% (v/v) 2-mercaptoethanol, 0.1 mM benzamidine, and 0.2 mM PMSF.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?